Production of live fish derived from frozen germ cells via germ cell transplantation

Sertoli cell condition medium can induce germ like cells from bone marrow derived mesenchymal stem cells

Objective(s): Although many researchers have confirmed induction of germ cells from bone marrow mesenchymal stem cells (BMMSCs), there are no reports that confirm spontaneous differentiation of germ cells from BMMSCs. In this study, we have evaluated the effect of adult Sertoli cell condition medium (SCCM) as a mutative factor in the induction of germ cells from BMMSCs. Materials and Methods: ...

In Vitro Production of Germ Cells from Stem Cells: Hypes and Hopes

Several lines of evidence have reported that mouse ESCs can successfully differentiate into primordial germ cells (PGC) as well as into mature male and female gametes. Human ESCs and adult stem cells (ASCs) can also differentiate into PGCs. Differentiation of ESCs into germ cells of various stages seems to be a spontaneous and quick process, probably due to the nature of ESCs themselves and the...

Production of the medaka derived from vitrified whole testes by germ cell transplantation

The medaka (Oryzias latipes) is a teleost model distinguished from other model organisms by the presence of inbred strains, wild stocks, and related species. Cryopreservation guarantees preservation of these unique biological resources. However, because of their large size, cryopreservation techniques for their eggs and embryos have not been established. In the present study, we established a m...

Germ cell transplantation in fish: basic biology and biotechnological applications

Surrogate Production of Eggs and Sperm by Intrapapillary Transplantation of Germ Cells in Cytoablated Adult Fish

Germ cell transplantation (GCT) is a promising assisted reproductive technology for the conservation and propagation of endangered and valuable genetic resources. In teleost fish, GCT in adult gonads has been achieved only in male recipients, limiting greatly the usefulness of this technique in situations where both sexes need equal and timely attention for conservation and/or propagation. Here...